1. 研究领域
单正链RNA病毒（Positive-strand RNA viruses）囊括大概1/3的病毒种类，包括许多对人类非常重要的病原，比如HAV, HEV, Zika virus, Dengue virus, HRV，EV71, Norovirus, SARS-CoV-2等。我们致力于研究单正链RNA病毒的复制机制，利用最新的病毒反向遗传学，生物化学，细胞生物学及结构生物学等手段，解析病毒复制复合体（repliase）组装的分子机制及病毒－宿主相互作用机制，以期了解病毒复制的新机制、找到新的抗病毒靶点以及新的疫苗策略。
2. 近年来科研成果（论文、专利、获奖等）
Selected publications (* corresponding author):
Zhang Y, Song W, Chen, S, Yuan Z*, Yi, Z*. A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2. Antiviral Res. In revision.
Yi Z#*, Ling Y#, Zhang X#, Chen J#, Hu K, Wang Y, Song W, Ying T, Zhang R, Lu H*, Yuan Z*. Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population. Emerg Microbes Infect. 2020 Aug 26:1-34 (#, co-first authors)
Zhang X#, Tan Y#, Ling Y#, Lu G#, Liu F#, Yi Z#, Jia X, Wu M, Shi B, Xu S, Chen J, Wang W, Chen B, Jiang L, Yu S, Lu J, Wang J, Xu M, Yuan Z, Zhang Q, Zhang X, Zhao G, Wang S*, Chen S*, Lu H*. Viral and Host Factors Related to the Clinical Outcome of COVID-19. Nature. 2020, 583(7816): 437-440 (#, co-first authors)
Yan J, Wang M, Wang M, Dun Y, Zhu L, Yi Z*, Zhang S*. Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species. Virus Res. 2020, 283: Apr 11:197974. doi: 10.1016/j.virusres.2020.197974.
Zhang H, Song Z, Zou J, Feng Y, Zhang J, Ren L, Zhang X, Hu Y, Yuan Z*, Yi Z*. An infectious clone of enterovirus 71(EV71) that is capable of infecting neonatal immune competent mice without adaptive mutations. Emerg Microbes Infect. 2020, 9(1):427-438.
Zhang Y, Zhao X, Zou J, Yuan Z*, Yi Z*. Dual role of the amphipathic helix of hepatitis C virus NS5A in the viral polyprotein cleavage and replicase assembly. Virology. 2019; 535: 283-296
Zhang Y, Zou J, Zhao, X, Yuan Z*, Yi, Z*. Hepatitis C virus NS5A inhibitor daclatasvir allosterically impairs NS4B-involved protein-protein interactions within the viral replicase and disrupts the replicase quaternary structure in a replicase assembly surrogate system. J Gen Virol. 2019;100(1):69-83
Du X, Zhang Y, Zou J, Yuan Z*, Yi, Z*. Replicase-mediated shielding of the poliovirus replicative double-stranded RNA to avoid recognition by MDA5. J Gen Virol. 2018;99(9):1199-1209
Zhang H, Song Z, Yu H, Zhang X, Xu S, Li Z, Li J, Xu H, Yuan Z, Ma H, Yi Z*, Hu Y. Genome-wide linear B-cell epitopes of enterovirus 71 in a hand, foot and mouth disease (HFMD) population. J Clin Virol. 2018;105:41-48
Du X, Pan T, Xu J, Zhang Y, Song W, Yi Z*, Yuan Z*. Hepatitis C virus replicative double-stranded RNA is a potent interferon inducer that triggers interferon production through MDA5. J Gen Virol. 2016 Nov;97(11):2868-2882.
国内发明专利：
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寨卡病毒MR766毒株的感染性克隆及其应用，易志刚, 袁正宏。2018101322778 (2018,2,9)
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基于EV71毒株的感染性cDNA克隆及其应用, 易志刚，宋志刚，张慧英，袁正宏。2019104740883 （2019,6,2）
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病毒SARS-CoV-2的特异性线性优势表位及对应多肽及其制药用途，易志刚, 袁正宏。202010665490.2（2020,7,11）
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基于细菌人工染色体的新冠病毒SARS-CoV-2亚基因组复制子克隆、构建及应用, 易志刚, 袁正宏。202010940386.X (2020,9.9)
3. 在研科研项目
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国家科技重大专项课题（2017－2020）：上海地区重点RNA病毒的变异变迁规律研究 （2017ZX10103009-004）
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国家自然科学基金面上项目(2018－2021): 宿主AAA+ATPase p97/VCP作为复制复合体重要组成参与单正链RNA病毒复制共同机制的研究（81772181）
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国家自然科学基金面上项目(2020－2023)：丙型肝炎病毒NS5A两性alpha-螺旋调控病毒复制复合体组装的机制研究（81971926）
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上海市科委应急项目（2020－2021）：上海市新型冠状病毒感染的肺炎流行病学研究 （20411950100）